2 edition of characterization of mumps virus-infected cell RNA. found in the catalog.
characterization of mumps virus-infected cell RNA.
Joseph Alphonsus Curran
Written in English
Thesis (Ph.D.)--The Queen"s University of Belfast, 1984.
|The Physical Object|
Fungal viruses (mycoviruses) have attracted more attention for their possible hypovirulence (attenuation of fungal virulence) trait, which may be developed as a biocontrol agent of plant pathogenic fungi. However, most discovered mycoviruses are asymptomatic in their hosts. In most cases, mycovirus hypovirulent factors have not been explored clearly. In this study, we characterized a ssRNA. RNA tumor virus-specific DNA in cells can be detected by its capacity to 1) alter the reassociation kinetics of labeled double-stranded product of viral RNA-directed DNA polymerase; 2) anneal single-stranded DNA (cDNA) synthesized by viral polymerase; or 3) hybridize labeled viral 70S (genomic) RNA. Duplexes formed with these procedures can be analyzed for fidelity of base pairing, and the Cited by:
Ebola virus (EBOV) glycoprotein (GP)–specific T-cell responses found in CD8 + T cells. A, EBOV GP–specific interferon γ (IFN-γ)/tumor necrosis factor α (TNF-α) T-cell responses. At left, graphs depict the observed IFN-γ/TNF-α responses in CD8 + (upper graph) and CD4 + (lower graph) compartments specific for the 4 EBOV GP peptide Cited by: Abstract. mRNA isolated from mumps virus-infected Vero cells was converted into cDNA and cloned into the PstI site of the plasmid pBR After screening with 32p-labelled cDNA synthesized from poly(A) + RNA of uninfected or mumps virus-infected Vero cells, Author: Narayanasamy Elango and Tamas M. Varsanyi.
Mumps virus is a member of the Paramyxoviridae family of enveloped, non-segmented, negative-sense RNA viruses. The signs of mumps include swelling of the parotid glands, salivary glands and other epithelial tissues. Symptoms of mumps are fatigue, body aches, headache, loss of appetite, low grade fever, swelling of the salivary : Monjiviricetes. Leydig cell production of mRNA and protein for interferons (IFNs) and of three antiviral proteins—2′5′ oligoadenylate synthetase (2′5′OAS), double-stranded RNA-activated protein kinase (PKR) and MxA—was investigated, in the absence or presence of mumps virus or viral stimuli including poly I:C, a mimetic of RNA viruses replication Cited by:
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Mumps virus is a nonsegmented, negative stranded RNA virus of the family Paramyxoviridae, subfamily Paramyxovirinae, genus Rubulavirus that causes mumps disease. Mumps virions are pleomorphic particles ranging from to nm in size [ 1 ], consisting of a helical ribonucleocapsid core surrounded by a host cell-derived lipid by: 5.
By the use of lysolecithin-permealized extracts from mumps virus-infected HeLa cells, we have developed an in vitro system, which not only directed the synthesis of mumps virus mRNAs but also supported replication of the genomic RNA.
Furthermore, upon transcription of the P gene, both faithful and edited copies of the P gene were detected by RNase mapping with a by: should be RNA extracted from cells infected with mumps virus. It is best to use cells infected with a well-characterized strain of mumps, belonging to a genotype not associated with the outbreak.
In addition, the use of vaccine virus as a control is not recommended since in an outbreak setting some cases may be referred for testing who. Mitogen-activated protein kinases (MAPKs) are the key participants in the determination of death and survival of virus-infected cells.
Akt, p90 ribosomal S6 kinase (p90RSK), and signal transducers for the activation of transcription 3 (STAT3) signaling pathways involve phosphorylation and dephosphorylation by viral infection. The mumps virus (MuV) V protein was characterized in virus infected cells by the use of antipeptide sera.
In radioimmune precipitation assay (RIPA), the sera reacted with the V protein and also immunoprecipitated the nucleocapsid (NP) and phospho (P) by: 2. Mumps virus replication was examined in various culture cells derived from mice. Eight of 16 lymphoid cell lines and 4 of 13 non-lymphoid cell lines supported the replication of Vero cell-adapted.
Mumps virus (MuV) belongs to the genus Rubulavirus in the family Paramyxoviridae, is a non-segmented, single-stranded, negative sense RNA virus that conta nucleotides in length. It encodes seven tandemly linked transcription units: the nucleo-(N), V/phosphor-/I (V/P/I), matrix (M), fusion (F), small hydrophobic (SH), hemagglutinin-neuraminidase (HN), and large (L) proteinsCited by: 1.
Mumps incidence in mainland China remains at a high level. Genotype F has been the predominant genotype of mumps virus (MuV) in the last 20 years in mainland China. To better understand the Cited by: 4. Mumps Virus RNA, Qualitative Real-Time PCR.
Test Code. CPT Code(s) CPT Code is subject to a Medicare Limited Coverage Policy and may require a. Mumps virus belongs to the family of Paramyxoviridae and has the potential to be an oncolytic agent. Mumps virus Urabe strain had been tested in the clinical setting as a treatment for human cancer four decades ago in Japan.
These clinical studies demonstrated that mumps virus could be a promising cancer therapeutic agent that showed significant antitumor activity against various types of by: 8. Mumps. Mumps virus infections are diagnosed by detecting (1) mumps virus in saliva, throat washings, or CSF; (2) mumps virus-specific IgM in serum specimens; (3) fourfold or greater elevations in mumps virus-specific IgG titers in acute and convalescent sera; or (4) mumps virus RNA in CSF or buccal swabs by using RT-PCR (Hatchette et al., ).
After screening with 32p-labelled cDNA synthesized from poly(A) + RNA of uninfected or mumps virus-infected Vero cells, five different groups of virus-specific clones were obtained. The virus specificity of the clones was confirmed by Northern blot analysis, in which the cDNA inserts from the five different groups hybridized to mRNAs of about,and nucleotides.
Molecular Biology of RNA Tumor Viruses deals with the molecular biology and biologic significance of RNA tumor viruses.
Methods and procedures with broad application to diverse areas of molecular biology, including cell culture procedures, competition radioimmunoassays, molecular hybridization, oligonucleotide mapping, heteroduplex mapping, and restriction endonuclease Book Edition: 1.
The MV V protein (40 kDa) was detected in MV-infected cells in a diffuse cytoplasmic distribution, a predominant subcellular localization distinct from that of virus nucleocapsids. Mumps was first described by Hippocrates in the fifth century bc, in his first Book of Epidemics, but a viral aetiology was not demonstrated until the s, when Johnson and Goodpasture fulfilled Koch's postulates by transferring the disease from experimentally infected rhesus macaques (Macaca mulatta), to children in his neighbourhood, using Cited by: A rapid, sensitive, and specific assay to detect mumps virus RNA directly from clinical specimens using a real‐time PCR assay was developed.
The assay was capable of detecting five copies of standard plasmid containing cDNA from the mumps virus F by: Purchase Nonsegmented Negative Strand Viruses - 1st Edition. Print Book & E-Book. ISBNCharacterization of Polycistronic Transcripts in Newcastle Disease Virus-Infected Cells Characterization of Structural Proteins of Parainfluenza Virus 3 and mRNAs from Infected Cells Characterization of Mumps Virus Book Edition: 1.
A prominent characteristic of virus-infected lungs was antigen in plump alveolar cells and desquamation of these cells into the alveolar Cited by: Virus infection in mammals elicits a variety of defense responses that are initiated by signals from virus-sensing receptors expressed by the host.
These receptors include the ubiquitously expressed RIG-I–like receptor (RLR) family of RNA helicases. RLRs are cytoplasmic proteins that act in cell-intrinsic antiviral defense by recognizing RNAs indicative of virus by: The duration of mumps virus RNA detection was studied during a mumps outbreak in a highly vaccinated university population.
Seven of the eight reverse transcription-PCR-positive specimens were collected during the first 3 days of parotitis, suggesting that viral shedding is minimal after the first 3 days of by:. The envelope of the mumps virus is a lipid bilayer, similar to the cell membrane that surrounds human cells.
In fact, the lipids found in the viral envelop are derived from the host cell.analysis was established. The study also allowed characterization of mumps virus strains from Argentina as part of a new subgenotype.
This PCR assay for characterization of mumps infections coupled to a web-based analytical program provides a rapid method for identiﬁcation of known and novel strains.
Mumps virus (MV), in the genus Rubulavirus.Mumps virus is a paramyxovirus in the same group as. parainfluenza and Newcastle disease virus. Parainfluenza and Newcastle disease viruses produce antibodies that cross-react with mumps virus. The virus has a single-stranded RNA genome.
The virus can be isolated or propagated in cultures of various human and monkey tissues and in embryonated File Size: KB.